SourceChickens were immunized with highly purified Lethal Factor A purified from Bacillus anthracis. After multiple immunizations in Freund"s adjuvant, eggs were collected and the IgY purified. Anti-Bacillus anthracis Lethal Factor A was immunoaffinity purified using the immunogen immobilized on a solid support.

Product

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Purification Method: Egg-yolk derived IgY Affinity Purified

Concentration: See vial for concentration.

ApplicationsThis antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:10,000 dilution of the IgY was an effective probe for 16 ng of Lethal Factor A. By Direct ELISA, the half-point titre was 1:38,000 dilution using a 2 ug/ml coating of Lethal Factor A. Optimal conjugate working dilutions should be determined for your particular assay conditions.

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf