| ProductType | ControlLysate |
| Units | 100µl |
| Application | WesternBlotWesternBlotting |
BackgroundTotalcellIysatesareusefulasbothpositiveandnegativecontrolsinimmunoblotting.A431,ahumanepidermoidcarcinomacellline,andtheEGF-stimulatedA431Iysatesareusedasnegativeandpositivecontrols,respectively,whenstudyingthephosphorylationcascadeinitiatedbyligandbindingtoreceptortyrosinekinases.BothA431andnormalhumanfibroblastsarevaluablepositivecontrolsforantibodiestoproteinsexpressedinthesecells.Themousefibroblastcellline3T3anditsRSV-transformedcounterpartserveasnegativeandpositivecontrols,respectively,forantibodiestoproteinsthatarephosphorylatedasaresultoftransformationbyRSV(Roussarcomavirus).MADIn-Darbybovinekidney(MDBK)cellIysatedisplaysproteinsexpressedinbovinekidneyandPC12(ratphaeochromocytoma)cellIysatecanbeexpectedtomirrortheproteinexpressionofcellsandtissuesofneuroectodermalorigin.Likewise,JurkatcellsexpresstheproteinsuniquetohumanT-cells.Ratbrainisawidelyusedpositivecontrolforthoseproteinsthatareexclusivelyexpressedinbrain.AtrightisanexampleoftheuseofpositiveandnegativecontrolIysatesinwesternblotting.Theblotof3T3cellIysate(lane1)andRSV-transformed3T3(lane2)wasprobedwithanti-phosphotyrosineperoxidaseconjugatetorevealthoseproteinsthataretyrosine-phosphorylatedinresponsetotransformationby
Synonyms:NormalHumanFibroblastcelllysate
Product
ProductForm:Unconjugated
Formulation:ProvidedassolutioninLaemmlielectrophoresissamplebuffer.Samplesneedtobeboiledfor3-5minutes,beforeloadingthegel.
Concentration:Seevialforconcentration
ApplicationsSuggesteduse:Avolumeof10-25ulissuggestedforimmunobloting.
FunctionalAnalysis:WesternBlotting
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butExalphaBIOLOGicalsacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:O00622
SpeciesAccession:Human
